动物营养学报2012，Vol.24Issue(12)：2424-2435,12.DOI:10.3969/j.issn.1006-267x.2012.12.017
绝食法与无氮饲粮法测定黄羽肉鸡内源性氨基酸排泄量及变异的比较研究
A Comparative Study of Fasting Method and Nitrogen-Free Diet Method for Determining Endougenous Amino Acid Excretion in Yellow-Feathered Chickens
摘要
Abstract
This experiment was conducted to compare the excretion and variation of endogenous amino acids (EAA) in yellow-feathered chickens under fasting and nitrogen-free diet (NFD) to evaluate the methods for the EAA determination in the measurement of the digestible amino acids of ingredients. Using a single factor completely randomized design, a total of 72 adult roosters aged 15 weeks were randomly selected according to their initial body weight and divided into 3 groups with 6 replicates per group and 4 roosters per replicate. The same experiment was repeated 4 batches to determine the EAA excretion under fasting, 25 g NFD, and 40 g NFD, respectively. The results showed as follows; the EAA excretion was increased significantly from the groups of fasting, 25 g NFD to 40 g NFD (P <0. 05). In the fasting group, the total coefficient of variation (CVtotal) , intragroup coefficient of variation ( CVwithin) , and intergroup coefficient of variation (CVbetween) of the EAA excretion of 17 amino acids among among 4 batches were from 9. 74% to 19. 99% , from 9. 97% to 16. 88% , and from2. 58% to 16. 23% , respectively. The differences of EAA excretion of threonine, cystine, serine and proline among 4 batches were significant (P < 0. 05). In the 25 g NFD group, the CVtotal, CVwithin, and CVbetween of the EAA excretion of 17 amino acids among 4 batches were from 17. 77% to 31. 33% , from 14. 99% to 28. 80% , and from 3. 89% to 17. 04% , respectively. The differences of EAA excretion of argi-nine, glycine and proline among 4 batches were significant (P <0. 05). In the 40 g NFD group, the CVtotal, CVwithin, and CVbetween of the EAA excretion of 17 amino acids among 4 batches were from 16. 23% to 33. 39% , from 17.15% to 33. 82% , and from 4. 19% to 16. 29% , respectively. The differences of EAA excretion of isoleucine and phenylalanine among 4 batches were significant (P <0. 05). It can be concluded that the excretion of EAA increases with the increasing amount of force-fed diet. The variation coefficients of the 25 and 40 g force-fed group are similar, and are both larger than those of fasting group.关键词
内源性氨基酸/绝食法/无氮饲粮法/黄羽肉鸡Key words
endogenous amino acids/ fasting method/ nitrogen-free diet method/ yellow-feathered chicken分类
农业科技引用本文复制引用
任立芹,赵峰,谭会泽,张建智,米宝民,赵江涛,张宏福..绝食法与无氮饲粮法测定黄羽肉鸡内源性氨基酸排泄量及变异的比较研究[J].动物营养学报,2012,24(12):2424-2435,12.基金项目
国家自然科学基金项目(30901037) （30901037）
北京市科技新星计划项目(2011098) （2011098）
科技部创新方法工作专项(2009IM033100) （2009IM033100）
