南方医科大学学报2012，Vol.32Issue(12)：1695-1698,4.DOI:10.3969/j.issn.1673-4254.2012.12.003
阿仑膦酸盐对成骨细胞相关基因RANKL、OPG表达的影响
Effect of alendronate on expressions of osteoprotegerin and receptor activator of nuclear factor κB ligand in mouse osteoblasts
摘要
Abstract
Objective To investigate the effect of alendronate on the expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in mouse osteoblasts. Method Mouse calvarial osteoblasts cultured in vitro were identified by alkaline phosphatase (ALP) staining and immunofluorescence assay of OPG and RANKL expressions. The second passage of the osteoblasts were treated with different concentrations of alendronate (10-4 to 10-7 mol/L) for 48 h, and the changes in OPG and RANKL mRNA and protein expressions were examined using real-time PCR and Western blotting, respectively. Results The isolated osteoblasts were positive for ALP and expressed OPG and RANKL. Real-time PCR and Western blotting showed that at the concentration of 1×10-4 mol/L, alendronate caused an obvious down-regulation of OPG and RANKL expressions in the cells, whereas at lower concentrations, alendronate increased the expressions of both genes with the highest expressions occurring after treatment with 1×10-5 mol/L. Conclusion High concentrations of alendronate (>1× 10-4 mol/L) decrease the expressions of OPG and RANKL, whereas low concentrations (1×10-5 to 1×10-7 mol/L) increase their expressions in mouse osteoblasts cultured in vitro.关键词
成骨细胞/阿仑膦酸盐/碱性磷酸酶/核因子κB受体激动剂配体/骨保护素Key words
osteoblasts/alendronate/alkaline phosphatase/receptor activator of nuclear factor ΚB ligand/osteoprotegerin分类
医药卫生引用本文复制引用
董伟,戚孟春,邓久鹏,陈洪伟,冯晓洁,廖囡囡..阿仑膦酸盐对成骨细胞相关基因RANKL、OPG表达的影响[J].南方医科大学学报,2012,32(12):1695-1698,4.基金项目
国家自然科学基金(81270965) （81270965）
河北省自然科学基金(C2011401044) （C2011401044）
河北联合大学科学研究基金(z201233) （z201233）
