南方医科大学学报2012，Vol.32Issue(12)：1722-1726,5.DOI:10.3969/j.issn.1673-4254.2012.12.009
小鼠慢性病毒性肝炎诱发肝脂肪变性模型的构建
Establishment of a murine model of hepatic steatosis induced by chronic viral hepatitis
摘要
Abstract
Objective To establish a animal model of hepatic steatosis induced by chronic viral hepatitis in C57BL/6 mice. Methods C57BL/6 mice were randomly assigned to control group, high-fat diet group, mouse hepatitis virus strain A59 (MHV-A59) virus infection group, and high-fat diet plus virus infection group. At 13 weeks of the experiment, serum samples were collected to detect MHV antibodies and transaminase and lipid levels. The hepatic pathologies of the mice were examined with Oil red O staining of the frozen sections the and HE staining of paraffin-embedded sections. Results The mice in the two virus infection groups showed strong positivity of MHV antibodies in the serum. Compared with the control group, the mice in high-fat diet group and the two virus infection groups had significantly increased AST and ALT levels with also elevated TC and LDL-C levels. The two virus infection groups both exhibited obvious pathologies in the liver characteristic of chronic viral hepatitis with increased lipid accumulation in the hepatocytes. Conclusion We have successfully established a mouse model of hepatic steatosis induced by chronic viral hepatitis, which provides the basis for further study of the disease mechanism.关键词
MHV-A59/慢性病毒性肝炎/肝脂肪变性/动物模型Key words
MHV-A59/chronic viral hepatitis/hepatic steatosis/animal model分类
医药卫生引用本文复制引用
甘露,张哲,郭进强,谢茜,蒙子君,万为人,罗炳德..小鼠慢性病毒性肝炎诱发肝脂肪变性模型的构建[J].南方医科大学学报,2012,32(12):1722-1726,5.基金项目
国家自然科学基金(31070687) （31070687）
广东省自然科学基金(9451051501002535) （9451051501002535）
南方医科大学公共卫生与热带医学学院学生课外科研项目(GWXS20110231) （GWXS20110231）
