南方医科大学学报2012，Vol.32Issue(12)：1792-1795,4.DOI:10.3969/j.issn.1673-4254.2012.12.023
罗格列酮对RAW264.7巨噬细胞源性泡沫细胞内胆固醇含量及SR-BⅠ表达的影响
Effects of rosiglitazone on cholesterol contents and scavenger receptor class B type Ⅰ expression in RAW264.7 foam cells
摘要
Abstract
Objective To observe the effect of rosiglitazone on the content of cholesterol and expressions of Acy-conzyme A: cholesterol acytransferase 1 (ACAT-1) and scavenger receptor class B type I (SR-BI) in RAW264.7 macrophage-derived foam cells and explore the anti-atherosclerotic mechanism of rosiglitazone. Methods RAW264.7 macrophages were incubated with oxidized low-density lipoproteins (ox-LDL) or with both ox-LDL and rosiglitazone (5,10, or 20 μmol/L). Oil red O staining was used to observe the formation of foam cells, and cholesterol oxidase was used to determine the content of cellular cholesterol contents. Western blotting was used observe the expressions of ACAT-1 and SR-BI in RAW264.7 foam cells. Results Compared with the control cells, RAW264.7 macrophage-derived foam cells showed significantly increased contents of total cholesterol and free cholesterol (P<0.01) and ACAT-1 expressions (P<0.05) with mildly increased SR-BI expression (P>0.05). Rosiglitazone treatments significantly lowered the contents of total cholesterol and free cholesterol (P<0.05), decreased the expression of ACAT-1 (P<0.05), and increased SR-BI expression (P<0.05) in the foam cells in a dose-dependent manner. Conclusion Rosiglitazone can decrease the contents of total and free cholesterol, down-regulate ACAT-1 expression and up-regulate SR-BI expression in the foam cells produce the anti-atherosclerotic effect.关键词
罗格列酮/泡沫细胞/胆固醇/酰基辅酶A-胆固醇酰基转移酶1/B族Ⅰ型清道夫受体Key words
rosiglitazone/foam cells/cholesterol/Acy-conzyme A: cholesterol acytransferase 1/scavenger receptor class B type I分类
医药卫生引用本文复制引用
徐芳,蒙颖,王志禄,李万玲,贾军正,郭文芬,谢宛霞,胡海英,胡旭堂..罗格列酮对RAW264.7巨噬细胞源性泡沫细胞内胆固醇含量及SR-BⅠ表达的影响[J].南方医科大学学报,2012,32(12):1792-1795,4.基金项目
甘肃省自然科学基金(1010RJZA122) （1010RJZA122）
甘肃省卫生行业科研计划管理项目(GWGL2010-16) （GWGL2010-16）
