南方医科大学学报2012，Vol.32Issue(12)：1828-1832,5.DOI:10.3969/j.issn.1673-4254.2012.12.032
环巴胺对人食管癌EC109细胞转移的影响及作用机制
Inhibitory effect of cyclopamine on metastatic ability of EC109 cells and its mechanism
摘要
Abstract
Objective To investigate the effect of cyclopamine on metastatic ability of human esophageal cancer EC109 cells and explore the possible mechanism. Methods Transwell chamber assay and angiogenesis assay were used to examine the metastatic ability, invasiveness and angiogenesis of EC109 cells treated with cyclopamine for 48 h. The expression of Gli-1 mRNA was detected using RT-PCR, and Western blotting was used to examine the protein expressions of Gli-1, matrix metalloproteainse-9 (MMP-9) and vascular endothelial growth factor (VEGF). Results Inhibition of the hedgehog signaling pathway by cyclopamine suppressed the migration, invasion, and angiogenesis of EC109 cells. Cyclopamine treatment significantly lowered the expression of Gli-1 mRNA (P<0.05) and the protein expressions of Gli-1, MMP-9 and VEGF (P<0.05). Conclusion Cyclopamine can significantly inhibit the metastatic capacity of EC109 cells possibly by down-regulating MMP-9 and VEGF expression as a result of Gli-1 inhibition.关键词
Hedgehog通路/环巴胺/EC109细胞系/转移Key words
hedgehog pathway/cyclopamine/EC109 cells/metastasis分类
医药卫生引用本文复制引用
左小平,秦治明,王凯斌,郑相如,陈立前..环巴胺对人食管癌EC109细胞转移的影响及作用机制[J].南方医科大学学报,2012,32(12):1828-1832,5.基金项目
重庆市卫生局重点课题基金(20121015) （20121015）
