南方医科大学学报2013，Vol.33Issue(2)：207-211,5.DOI:10.3969/j.issn.1673-4254.2013.02.10
槲皮素对胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响
Effect of quercetin on invasion, migration, proliferation and cell cycle of glioma U87 cells
摘要
Abstract
Objective To explore the effect of quercetin on the invasion, migration, proliferation and cell cycle of glioma U87 cells. Methods Glioma U87 cells were treated with 50,100, or 150 μmol/L quercetin (Q50, Q100 and Q150 groups, respectively) or with DMSO (Q0 group). Transwell in vitro invasion and migration assays, Click-iT Edu test and flow cytometry were performed to evaluate the effect of quercetin on the invasion, migration, proliferation and cell cycle of U87 cells. Results After 36 h of quercetin treatment, the cells in Q50, Q100 and Q150 groups showed invasive cell percentages (relative to Q0 group) of 52.08%, 24.63%, and 13.13%, respectively (P<0.05). After quercetin treatment for 12 h, the migrating cell percentages (relative to Qo group) in Q50, Q100 and Q150 groups were 49.46%, 26.78%, and 14.56%, respectively (P<0.05). After 24 h of quercetin treatment, the cell proliferation ratios in Q0, Q50, Q100and Q150 groups were 25.21%, 18.38%, 16.74% and 15.24%; the cell percentages in phase G0/G1 were 71.14%, 72.71%, 69.29%, and 66.47%, phase S were 25.32%, 22.48%, 21.96%, and 23.32%, and phase G2/M were 3.53%, 4.80%, 8.75%, and 10.25% in the 4 groups, respectively, showing a significant difference between groups Q100, Q150 and group Q0 in phase G2/M cell percentages (P<0.05). Conclusion Quercetin can significantly inhibit the invasion, migration and proliferation of glioma U87 cells by blocking the cell cycle progression.关键词
槲皮素/U87胶质瘤细胞/侵袭/迁移/增殖/细胞周期Key words
quercetin/glioma U87 cells/cell migration/invasion/proliferation/cell cycle引用本文复制引用
苑召虎,胡子有,张兰兰,颜晓慧,王惠丽,吴炳义..槲皮素对胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响[J].南方医科大学学报,2013,33(2):207-211,5.基金项目
国家863子课题(2006AA020701) （2006AA020701）
