南方医科大学学报2013，Vol.33Issue(2)：253-257,5.DOI:10.3969/j.issn.1673-4254.2013.02.20
HBeAg的B细胞线性表位预测及鉴定
Prediction and identification of B-cell linear epitopes of hepatitis B e antigen
摘要
Abstract
Objective To predict and identify B-cell linear epitopes of hepatitis B e antigen (HBeAg). Methods The B-cell linear epitopes of HBeAg were predicted using the software provided by NCBI Database and Immune Epitope Database (IEDB) and synthesized by a solid-phase method followed by conjugation with keyhole limpet hemocyanin (KLH). The KLH conjugates were used for immunization of New Zealand white rabbits, and the immune response of the rabbits was monitored by direct ELISA using a bovine serum albumin conjugate of the predicted epitopes. Results Four new B-cell linear epitopes of HBeAg were identified, namely 1MDIDPYKEFG10,37LYREALESPEHCSP50,74SNLEDPAS81 and 127RTPPAYRPPNAPIL140. The rabbits immunized with the KLH conjugate showed an antibody titer over 1:512 000. The antisera of B-cell linear epitopes collected could specifically react with HBeAg as shown by ELISA. Conclusion Four B-cell linear epitopes of HBeAg have been confirmed using bioinformatics methods, which provides new evidence for further functional studies of HBeAg in hepatitis B.关键词
乙型肝炎病毒/乙肝e抗原乙型肝炎病毒/抗原表位预测/B细胞线性表位Key words
hepatitis B virus/hepatitis B e antigen/antigen epitopes/B-cell linear epitopes引用本文复制引用
杨军,刘妮,张婷,赵世平,强磊,苏宝山,康安静,李宗芳..HBeAg的B细胞线性表位预测及鉴定[J].南方医科大学学报,2013,33(2):253-257,5.基金项目
陕西省科技统筹创新工程计划项目(2011KTCL03-14) （2011KTCL03-14）
教育部新教师基金(2007-0698106) （2007-0698106）
教育部新世纪优秀人才计划(NCET-10-0647) （NCET-10-0647）
