南方医科大学学报2013，Vol.33Issue(2)：305-308,4.DOI:10.3969/j.issn.1673-4254.2013.02.32
天然虾青素对抗肾纤维化及细胞凋亡的作用
Effects of astaxanthin on renal fibrosis and cell apoptosis induced by partial unilateral ureteral obstruction in rats
谢潮鑫 1孟猛 1殷先锋 1何凤玲 1叶汉深 2谢栋2
作者信息
- 1. 解放军第421医院肾内科,广东广州510310
- 2. 解放军第421医院医务处,广东广州510310
- 折叠
摘要
Abstract
Objective To study the effects of astaxanthin on renal fibrosis and apoptosis induced by partial unilateral ureteral obstruction (UUO) in rats. Methods Ninety-six male adult SD rats were randomized into 6 equal groups, namely the blank control group, sham-operated group, UUO group, and astaxanthin group at high, medium, and low doses. Left ureteral ligation was performed in UUO and astaxanthin groups, and two days before the operation, the rats in astaxanthin groups were lavaged with 25, 50, or 100 mg/kg astaxanthin daily for 14 days, while the same volume of saline was given to rats in UUO group and sham-operated group. Renal pathological in the rats was observed with HE staining, and the expression levels of TGF-β1, SGK1, and CTGF in the left kidney were detected immunohistochemically; the expression level of Bcl-2 and Bax were detected using Bcl-2 and Bax detection kits. Results Compared to UUO group, high- and medium-dose astaxanthin groups showed obviously ameliorated renal pathologies and reduced expressions of TGF-β1, SGK1, and CTGF in the left kidney with lessened renal cell apoptosis. Conclusion Astaxanthin can reduce UUO-induced renal fibrosis and renal cell apoptosis, demonstrating the renoprotective effect of astaxanthin against renal fibrosis.关键词
天然虾青素/单侧输尿管梗阻/肾纤维化/细胞凋亡Key words
astaxanthin/unilateral ureteral obstruction/renal fibrosis/cell apoptosis引用本文复制引用
谢潮鑫,孟猛,殷先锋,何凤玲,叶汉深,谢栋..天然虾青素对抗肾纤维化及细胞凋亡的作用[J].南方医科大学学报,2013,33(2):305-308,4.
