电子科技大学学报2012，Vol.41Issue(5)：796-800,5.DOI:10.3969/j.issn.1001-0548.2012.05.029
丹参SAMDC基因的电子克隆及序列分析
In Silico Cloning and Characterization of the SAMDC Gene in Salvia Miltiorrhiza Bge
摘要
Abstract
S-adenosyl-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In this paper, using bioinformatics strategy and validating by experiment, a novel SAMDC of Salvia miltiorrhiza Bge was cloned and identified which was being blasted by search of Salvia miltiorrhiza expressed sequencetags (EST) database with homologous gene cDNA of Nicotiana tabacum. This sequence was confirmed by RT- PCR, molecular cloning and sequencing. Sequence analysis showed that the Silvia miltiorrhiza SAMDC (SmSAMDC) consisted of three open reading frames (ORFs) (tiny ORF, small ORF andyfnain ORF), and the main ORF encoded 360 amino acids with a calculated molecular weight of 39.4 kD and isoelectric point of 4.71. Tertiary structure predicted that 25.28%, 24.17% and 50.56% amino acids composed a helix, extending strand and random coil, respectively. Alignments of deduced amino acid sequences demonstrated that the SmSAMDC had several highly conserved motifs in plant SAMDC family, including a putative pro-enzyme cleavage site a PEST sequence.关键词
电子克隆/多胺/S-腺苷甲硫氨酸脱羧酶/丹参Key words
in silico cloning/ polyamine/ S-adenosylmethionine decarboxylase/ Salvia miltiorrhiza分类
农业科技引用本文复制引用
邓科君,杜梅泽,卢戟,任正隆..丹参SAMDC基因的电子克隆及序列分析[J].电子科技大学学报,2012,41(5):796-800,5.基金项目
国家自然科学基金(30730065) （30730065）
