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香竹愈伤组织诱导研究

孙瑞明 王娟 陈芳 彭桂莎 杨宇明 徐田

福建林学院学报2013,Vol.33Issue(1):48-51,4.
福建林学院学报2013,Vol.33Issue(1):48-51,4.

香竹愈伤组织诱导研究

Study on callus inducement of Chimonocalamus delicates

孙瑞明 1王娟 1陈芳 2彭桂莎 1杨宇明 2徐田2

作者信息

  • 1. 西南林业大学,国家林业局西南生物多样性保育重点实验室,云南昆明 650224
  • 2. 云南省林业科学院,云南昆明 650204
  • 折叠

摘要

Abstract

Based on murashige and skoog (MS) medium, induction and proliferation of callus under different concentration of 2,4-D, KT, and 6-BA were used for the evaluation of the effect of callus. Seeds, sterile roots, sterile stems and sterile leaves were used as explants for evaluation of the callus induction efficacy of Chimonocalamus delicatus. The results showed that MS + 3.0 mg·L-12,4-D + 0.5 mg·L-1 6-BA was an optimum medium for callus inducing with different conditions. 1.0 mg·L-12,4-D coordinated with 1.0 mg·L-1 6-BA and 0.5 mg·L-1 KT in MS, were more suitable for callus proliferation of C. delicatus. The root cap could be easier to induce callus formation than stem and leaves. However, seeds are the most proper explants to induce the callus formation.

关键词

香竹/愈伤组织/诱导/增殖

Key words

Chimonocalamus delicatus/ callus/ induction/ proliferation

分类

生物科学

引用本文复制引用

孙瑞明,王娟,陈芳,彭桂莎,杨宇明,徐田..香竹愈伤组织诱导研究[J].福建林学院学报,2013,33(1):48-51,4.

基金项目

云南省自然科学基金资助项目(2009CD073) (2009CD073)

国家林业局"948"技术引进基金资助项目(2008-4-30) (2008-4-30)

云南省中青年学术技术带头人后备人才培养基金资助项目(2010CI016). (2010CI016)

福建林学院学报

OA北大核心CSCDCSTPCD

2096-0018

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