福建农林大学学报（自然科学版）2013，Vol.42Issue(1)：68-71,4.
采用荧光光谱法研究烟酸与牛血清白蛋白的相互作用
Studies on infraction between nicotinic acid and bovine serum albumin by fluorescence spectrometry
摘要
Abstract
The interaction between nicotinic acid (NTA) and bovine serum albumin (BSA) in physiological solution was investigated by fluorescence spectrometry. The results showed fluorescence intensity of BSA was quenched by NTA based on the dynamic quenching model. The binding constants were obtained by calculation, which were 31.9 (293 K) and 72.9 L·mol-1 (310 K) respectively. The thermodynamic parameters obtained from measured data showed that the interaction of NTA and BSA was mainly driven by hydrophobic force. Synchronous fluorescence spectrometry and ultraviolet (UV) spectroscopy indicated that NTA did not affect the conformation of BSA.关键词
荧光光谱/烟酸/牛血清白蛋白/紫外光谱Key words
fluorecense spectrometry/ nicotinic acid/ bovine serum albumin/ ultraviolet spectroscopy分类
化学化工引用本文复制引用
蔡向阳,谢勇平,蔡碧琼,郑新宇,李清禄..采用荧光光谱法研究烟酸与牛血清白蛋白的相互作用[J].福建农林大学学报（自然科学版）,2013,42(1):68-71,4.基金项目
福建省自然科学基金资助项目(B0410012). （B0410012）
