福建农业学报2012，Vol.27Issue(12)：1280-1282,3.
创伤弧菌外膜蛋白免疫刺激复合物最小免疫剂量测定
Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla
摘要
Abstract
Antigenicity of the immune stimulating complexes in the outer membrane protein (OMP-ISCOMs) of Vibrio vulnifiticus was studied by injecting the vaccine into the European eels (Anguilla anguilla). The minimum dosage required for effective vaccination was determined by serology and immune protection test. The immune response was dosage dependent. Thirty days after the eels were challenged with the V. vulnificus FJ03-X2 strains at 200, 100, 50 and 25 μg·mL-1 to result in 100%, 87. 5% , 75% and 50% of protection, respectively; and the titers of serum antibody of 1:6400, 1:3200, 1:800 and 1:200 in turn, respectively. It appeared that the minimum dose of the OMP-ISCOMs of Vibrio vulnifiticus vaccine was 0. 59 μg·g-1.关键词
创伤弧菌/OMP-ISCOMs/最小免疫剂量/欧洲鳗鲡Key words
Vibrio vulnifiticus/ OMP-ISCOMs/ minimum immune dose/ Anguilla anguilla分类
农业科技引用本文复制引用
许斌福,龚晖,陈秀锦,田丁,陈强,陈永聪,林天龙..创伤弧菌外膜蛋白免疫刺激复合物最小免疫剂量测定[J].福建农业学报,2012,27(12):1280-1282,3.基金项目
福建省农业科学院科技创新团队建设项目(STIF-Y02) （STIF-Y02）
福建省科技创新平台项目(2009N2003) （2009N2003）
国家自然科学基金项目(31101933) （31101933）
福建省农业科学院科研项目(工程化实验室类) （工程化实验室类）
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1021-5) （2010R1021-5）
