福建农业学报2012，Vol.27Issue(12)：1308-1312,5.
龙眼新品种(品系)的ISSR指纹图谱研究初探
DNA Fingerprinting of 17 Dimocarpus longan Germplasms by Using ISSR Markers
摘要
Abstract
The inter-simple sequence repeat (ISSR) method was applied to assess the genetic makeup of 17, including 6 new longan varieties.Out of the 30 ISSR primers, 5 were selected for the analysis. Cluster analysis showed that the genetic similarity coefficients ranged from 0. 75 to 0. 93, and that the 17 cultivars could be clustered into 4 groups using a genetic similarity coefficient threshold of 0. 785. These varieties could be satisfactorily distinguished by a single ISSR primer, py-9, and established their digital DNA fingerprints accordingly. The results provided an important basis for identifying new longan varieties, as well as evaluating and utilizing the germplasm resources.关键词
龙眼/ISSR/指纹图谱/遗传多样性Key words
Longan/ ISSR/ fingerprint/ genetic diversity分类
农业科技引用本文复制引用
郑姗,张立杰,张小艳,谢丽雪,李韬..龙眼新品种(品系)的ISSR指纹图谱研究初探[J].福建农业学报,2012,27(12):1308-1312,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1016-4) （2010R1016-4）
福建省自然科学基金项目(2012J01100) （2012J01100）
福建省农业科学院青年科技人才创新基金(2010QB-12) （2010QB-12）
