福建师范大学学报（自然科学版）2013，Vol.29Issue(1)：86-89,100,5.
甘蔗SPSⅢ启动子区ATCT-motif和CAT-box元件的酵母单杂交报告载体构建
Construction of Yeast One-hybrid Reporter Vector for Screening the Binding Proteins of ATCT-motif and CAT-box in SPS Ⅲ Promoter
摘要
Abstract
Sucrose phosphate synthase is the key enzyme for sucrose accumulation in plant, which contains two specific domain in its promoter region, light-response element ATCT-motif and meristem-specificity element CAT-box. To concretely analyze the function of ATCT-motif and CAT-box from sucrose phosphate synthase Ⅲ in sugarcane, the fragment containing these two elements from promoter region ( - 1 320 ～-1 210) was cloned and inserted into yeast one-hybrid bait vector to construct pAbAi-SPS Ⅲ-AB vector which then was transformed to yeast to screen the binding proteins. It provided a framework for further investigation on the regulation mechanism of SPS gene in sugarcane.关键词
酵母单杂交/蔗糖磷酸合成酶基因Ⅲ/转录因子/启动子Key words
yeast one-hybrid system/ sucrose phosphate synthase Ⅲ/ transcription factor/ promoter分类
生物科学引用本文复制引用
张积森,林清凡,方静平,邹丽娟,叶冰莹,陈由强,陈如凯..甘蔗SPSⅢ启动子区ATCT-motif和CAT-box元件的酵母单杂交报告载体构建[J].福建师范大学学报（自然科学版）,2013,29(1):86-89,100,5.基金项目
国家自然科学基金资助项目(31201260) （31201260）
农业部现代农业产业技术体系建设专项资金(CARS-20-4-4) （CARS-20-4-4）
福建省教育厅资助项目(JA12082) （JA12082）
