分析测试学报2013，Vol.32Issue(1)：84-88,5.DOI:10.3969/j.issn.1004-4957.2013.01.013
浓缩果汁中甲基硫菌灵、2-氨基苯并咪唑、多菌灵、噻菌灵及5-羟基噻菌灵残留量的液相色谱-串联质谱法测定
Simultaneous Determination of Thiophanate-methyl, 2-Aminobenzimidazole,Carbendazim, Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS
摘要
Abstract
A liquid chromatography tandem mass spectrometric method was developed for the determination of benzimidazole fungicides and their metabolites such as thiophanate-methyl (TM ) , 2-amino-benzimidazole(2-AB), carbendazim ( MBC) , triabendazole (TBZ), 5-hydroxy-triabendazole(5-OH-TBZ) in 7 types of concentrated fruit juices including apple, mango, pineapple, pear, orange, ly-chee and passion fruit. Seven representative concentrated fruit juices were selected and extracted with acetonitrile. The extracts were purified with PSA column. The separation of 5 analytes was carried out on a ZORBAX Eclipse XDB - C18 column using methanol - 10 mmol/L ammonium acetate(containing 0. 1% formic acid) as mobile phase by gradient elution. The detection of analytes was performed by electrospray tandem mass spectrometry with ES + and multiple reaction monitoring ( MRM) modes. The results showed that, the method showed good linear relationships in the range of 0. 02 -0. 2 mg/L for the tested samples, with correlation coefficients no less than 0. 998 5. The quantitation limits were 2. 0 (μg/kg for 2-AB and 5-OH - TBZ, and 1.0 μ,g/kg for the rest of the analytes. The mean recoveries ranged from 70% to 110% with relative standard deviations of 0. 1% -10. 9% when the fortified levels for 2-AB and 5-OH-TBZ were 2, 4, 20 μg/kg and those for TBZ, MBC, TM were 1, 2, 10 μg/kg, respectively. The proposed method was sensitive and reliable, and was successfully applied in the determination of benzimidazole fungicides and their metabolites in real commercial concentrated juices.关键词
LC-MS/MS/浓缩果汁/甲基硫菌灵/2-氨基苯并咪唑/多菌灵/噻菌灵/5-羟基噻菌灵Key words
liquid chromatography tandem mass spectrometry/ concentrated fruit juices/ thio- phanate-methyl/ 2-aminobenzimidazole/ carbendazim/ triabendazole/ 5-hydroxy-triabendazole分类
化学化工引用本文复制引用
刘晓松,郑玲,黄文雯,卢炜,宁恩创,方晓明..浓缩果汁中甲基硫菌灵、2-氨基苯并咪唑、多菌灵、噻菌灵及5-羟基噻菌灵残留量的液相色谱-串联质谱法测定[J].分析测试学报,2013,32(1):84-88,5.基金项目
国家质检总局科研计划项目(2010IK164) （2010IK164）
广西自然科学基金项目(2010GXNSFA013039) （2010GXNSFA013039）
