分析测试学报2013，Vol.32Issue(1)：106-111,6.DOI:10.3969/j.issn.1004-4957.2013.01.017
桑叶HILIC特征指纹图谱研究
Study on the Hydrophilic Interaction Chromatography Specific Fingerprint of Mulberry leaves
摘要
Abstract
A hydrophilic interaction chromatography (HILJC) fingerprinting method had been established for the quality evaluation and discrimination of mulberry leaves. The chromatographic fingerprints were determined by injecting 20 μL of the sample solution each time into the hydrophilic interaction chromatography column(XBridge? Amide column) with the gradient elution solvent system composed of water (containing 0. 2% formic acid, 20 mmol/L ammonium formate and 20% methanol) and acetonitrile. The flow rate was 0. 8 mL/min, the column temperature was maintained at 25℃ and the signals were acquired at 322 nm. The result indicated that the developed HILIC method was simple, accurate and reliable for the development of mulberry leaves fingerprint. The HILIC fingerprints for ten samples tested contained the same seventeen peaks and four characteristic peaks. Combining with the similarity analysis, the method could be used to discriminate different origins of mulberry leaf samples. The HILIC fingerprinting technique is expected to become an important tool in the identification and quality evaluation of mulberry leaves.关键词
桑叶/亲水色谱/指纹图谱Key words
poly (lactic-co-glycolic-acid)/ polyethyleneglycol/ aptamer/ nanoparticle/ gene vector分类
化学化工引用本文复制引用
东莎莎,赵恒强,王晓,崔清华,耿岩玲,刘代成,刘建华,王小如..桑叶HILIC特征指纹图谱研究[J].分析测试学报,2013,32(1):106-111,6.基金项目
国家重大新药创制(2011ZX09201-201-26) （2011ZX09201-201-26）
山东省科学院科技发展基金项目(2012) （2012）
山东省大型分析仪器升级改造项目(2012SJGZ01) （2012SJGZ01）
山东省科技攻关计划项目(2011GSF11908) （2011GSF11908）
