分析测试学报2013，Vol.32Issue(2)：150-155,6.DOI:10.3969/j.issn.1004-4957.2013.02.002
粮谷中8种痕量真菌毒素的定量分析方法
Accurate Quantitative Analysis Method for 8 Kinds of Mycotoxins in Cereal
摘要
Abstract
A high performance liquid chromatography - tandem mass spectrometric( HPLC - MS/MS) method was developed for the determination of 8 kinds of mycotoxins (aflatoxin Bl, aflatoxin B2, af-latoxin Gl, aflatoxin G2, fumonisin Bl, fumonisin B2, sterigmatocystin and roquefortine C) in ce real. The sample was diluted with hexane to remove the oil, then extracted with 60% acetonitrile. The acetonitrile layer was made up to constant volume and passed through the filter before tested. The detection was performed in the electrospray ion positive mode and multiple reaction monitoring (MRM) mode, and the quantitation of target compounds was carried out by the isotope dilution assays. The results indicated that the 8 compounds showed good linear relationships in certain concentration ranges, with correlation coefficients not less than than 0.997 0. The average recoveries ranged from 77% to 123%, with relative standard deviations of 0. 6% - 13. 3% . The method was proved to be rapid and sensitive, and could be used for the detection of mycotoxins in cereal.关键词
同位素内标稀释/粮谷/真菌毒素/液相色谱-串联四极杆质谱Key words
isotope dilution assay/ cereal/ mycotoxins/ high performance liquid chromatography -tandem mass spectrometry分类
化学化工引用本文复制引用
曹娅,孙利,王明林,冯峰,储晓刚..粮谷中8种痕量真菌毒素的定量分析方法[J].分析测试学报,2013,32(2):150-155,6.基金项目
中国检验检疫科学研究院基本科研业务费专项资金资助项目(2010JK001) （2010JK001）
