分析测试学报2013，Vol.32Issue(2)：223-227,5.DOI:10.3969/j.issn.1004-4957.2013.02.014
2,4-二硝基苯肼衍生/高效液相色谱法测定葡萄酒中总乙偶姻的含量
Determination of Total Acetoin in Wine after Derivatization with 2, 4-Dinitrophenylhydrazine by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatographic method was developed for the determination of total acetoin in wine using 2, 4-dinitrophenylhydrazine(DNPH) derivatization. The experimental con ditions, including pH value, reaction time, DNPH content, reaction temperature and processing step after derivatization, were optimized. The derivatization products were separated on an Ultimate C18 column (4. 6 mm x250 mm, 5 μm) using acetonitrile -water (55 : 45) as mobile phase at a flow rate of 1. 0 mL/min. The oven temperature was set at 30℃, and the detection wavelength was 363 nm. Under the optimal conditions, the linear range for acetoin was in the range of 0 -40. 0 mg/L with correlation coefficient of 0. 999 9. The spiked recoveries of acetoin in the dry red wine sample were in the range of 95% - 106% with relative standard deviations (RSDs) of 2. 3% - 3. 4%, and the spiked recoveries of the dry white wine samples were in the range of 94% - 104% with RSDs of 2. 5% - 3. 1% . The contents of acetoin in some different types of wine samples were tested, and the results showed that the concentrations of total acetoin in domestic fake and shoddy wine samples were much higher than those in good quality wine samples. Besides, the concentration of total acetoin in home-brewed wine samples from badgrapes were also higher. Therefore, the concentration of total acetoin can provide a reference for the wine quality identification.关键词
乙偶姻/二硝基苯肼/衍生/高效液相色谱/葡萄酒Key words
acetoin/ dinitrophenylhydrazine/ derivatization/ high performance liquid chromatogra- phy / wine分类
化学化工引用本文复制引用
余书奇,晏日安,陈文锐,奚星林,刘青,邵仕萍..2,4-二硝基苯肼衍生/高效液相色谱法测定葡萄酒中总乙偶姻的含量[J].分析测试学报,2013,32(2):223-227,5.基金项目
广东省科技计划项目(2010B020316006,2011B050400025) （2010B020316006,2011B050400025）
质检公益性行业科研专项(201210104-2) （201210104-2）
