分析测试学报2013，Vol.32Issue(2)：239-243,5.DOI:10.3969/j.issn.1004-4957.2013.02.017
光谱法研究根皮苷与人血清白蛋白相互作用
Study on Interaction between Phlorizin and Human Serum Albumin by Spectroscopic Methods
摘要
Abstract
The interaction of phlorizin with human serum albumin ( HSA) was studied by fluorescence spectrometry, UV -visible absorption spectrometry and synchronous fluorescence spectrometry. The results showed that phlorizin could induce an endogenous fluorescence quenching of HSA under a mechanism of static quenching. The binding constants were determined to be 3. 163 5 × 10 (293 K), 1.774 8 ×l05(303 K) and 1. 193 5 x105(313 K) L mol-1 respectively. The binding site(n) was about 1. The thermodynamic parameters suggested that the interaction forces between phlorizin and HSA were mainly hydrogen and vander waals force. The binding distance was estimated to be 3. 97 nm according to Forster nonradiation energy transfer mechanism. The results of synchronous fluorescence spectra of phlorizin - HSA system showed that phlorizin mainly interacted with tryptophan in HSA, leading to a local change in protein conformation. In addition, the addition of the common i-ons could affect the binding constant of phlorizin - HSA complex.关键词
根皮苷/人血清白蛋白/荧光猝灭/热力学参数Key words
phlorizin/ human serum albumin (HSA) / fluorescence quenching/ thermodynamic pa- rameter分类
化学化工引用本文复制引用
王迎进,张艳青,李亚雄,李艳芳,张海容..光谱法研究根皮苷与人血清白蛋白相互作用[J].分析测试学报,2013,32(2):239-243,5.基金项目
山西省留学回国人员科技活动择优资助项目(2010-97) （2010-97）
忻州师范学院科研资助项目(2009-54) （2009-54）
忻州师范学院大学生科技创新项目(2012) （2012）
