分析化学2012，Vol.40Issue(9)：1336-1340,5.DOI:10.3724/SP.J.1096.2012.11126
蛋白-脂质体复合物毛细管电泳筛选单胺氧化酶抑制剂
Screen of Monoamine Oxidase Inhibitors by Protein-Liposome Conjugate Capillary Electrophoresis
摘要
Abstract
Based on the interaction between enzyme and substrate, a simple and elegant method was developed to screen the inhibitors of monoamine oxidase (MAO). Four kinds of N-propargyl amine compounds with gradient concentrations were added in the CE running buffer containing protein-liposome conjugate to inhibit the activity of MAO to some extent. The relative migration time ratio (RMTR) of kynuramine (Kyn) was investigated in running buffer containing different compounds in gradient concentrations. The results showed that compound 1 and 2 could inhibit the activity of MAO obviously, such that the interaction between MAO and Kyn was weakened, RMTR of Kyn increased with the increase of compound 1 and 2. The inhibition activities of compound 3 and 4 were not strong, and RMTR of Kyn changed little with the increase of compound 3 and 4. This result was consistent with that of activity assay of compounds incubation offline. In comparison with the traditional screening method for MAO inhibitors, this method is simple, rapid, less consumption of enzyme and did not suffer from the interference caused by non-target molecules.关键词
脂质体/毛细管电泳/单胺氧化酶/抑制剂/相互作用Key words
Liposome/ Capillary electrophoresis/ Monoamine oxidase/ Inhibitors/ Interaction引用本文复制引用
李冰,吕雪飞,庆宏,邓玉林..蛋白-脂质体复合物毛细管电泳筛选单胺氧化酶抑制剂[J].分析化学,2012,40(9):1336-1340,5.基金项目
本文系国家自然科学基金(No.20905007),教育部新世纪优秀人才支持计划(No.NCET-08-0048)和国家重点基础研究发展规划(No.2009BAK59B01)资助项目 （No.20905007）
