分析化学2012，Vol.40Issue(9)：1421-1428,8.DOI:10.3724/SP.J.1096.2012.11185
给药硝酸镨后大鼠尿液和血清的核磁共振代谢组学研究
Nuclear Magnetic Resonance-based Metabonomic Studies on Urine and Serum from Pr(NO3)a-treated Rats
摘要
Abstract
The acute biochemical effects of Pr(NO3)3 were studied by NMR-based metabonomic approach on rats urine and serum. Male Wistar rats were administrated intraperitoneally with Pr(NO3)3 at doses of 2, 10 and 50 mg/kg body weight. Urine was collected over a 168 h period after dosing and serum was collected at 48, 96 and 168 h period. 1H NMR spectra of biofluids (urine and serum) were analyzed by principal components analysis. The results revealed that Pr(NO3)3 caused acute damages on rats liver and kidney, mainly in the liver, and showed a significant dose-dependent relationship. The liver and kidney injury induced by Pr(NO3)3 was characterized by the variations of many endogenous metabolites, such as citrate, succinate, α-ketoglutarate, creatinine, N-trimethyl dioxide, amino acids, lactate, taurine and glucose.关键词
硝酸镨/体液/核磁共振/生物效应/模式识别/代谢组学Key words
Praseodymium nitrate/ Biofluids/ Nuclear magnetic resonance/ Biochemical effects/ Pattern recognition/ Metabonomics引用本文复制引用
廖沛球,薛蓉,吴亦洁,裴奉奎,李晓晶..给药硝酸镨后大鼠尿液和血清的核磁共振代谢组学研究[J].分析化学,2012,40(9):1421-1428,8.基金项目
本文系国家自然科学基金资助项目(No.20575065和20975097) （No.20575065和20975097）
