分析化学2012，Vol.40Issue(10)：1589-1592,4.DOI:10.3724/SP.J.1096.2012.20324
顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物
Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction
摘要
Abstract
A headspace solid phase microextraction (HS-SPME), followed by gas chromatographic method was established for the detection of three pyrazine compounds (2,5-dimethyl pyrazine, 2,3,5-trimethyl pyrazine and 2,3, 5, 6-tetramethyl pyrazine) in cocoa wort. Equilibrium temperature and equilibrium time were optimized by using different SPME heads. According to the results of the optimization experiments, the following conclusion can be drawn: The cocoa wort sample was efficiently extracted under 40 ℃ for 40 min with 75 urn CAR/PDMS SPME head. The method had linear response within 1 - 500 mg/L, and the detection limits (S/N=3) were below 0. 023 礸/L. The relative standard deviations were from 3. 6%-6. 4% and the recoveries were from 95. A% to 102. 7%. This method is characterized by rapidly, high sensitivity, good linearity and repeatability for the analysis of cocoa wort samples.关键词
顶空-固相微萃取(HS-SPME)/吡嗪类物质/气相色谱/可可麦汁Key words
Headspace solid phase microextraction/ Pyrazine compounds/ Gas chromatography/ Cocoa wort引用本文复制引用
肖天鹏,郑晓峰,吕吉鸿,郭泽峰,高峰,刘春凤,李崎..顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物[J].分析化学,2012,40(10):1589-1592,4.基金项目
本文系国家"十一五"支撑重点项目(No.2008BAI63B06)资助 （No.2008BAI63B06）
