分析化学2012，Vol.40Issue(10)：1593-1597,5.DOI:10.3724/SP.J.1096.2012.20115
酸性红73多克隆抗体的制备及其应用
Preparation and Application of Acid Red 73 Polyclonal Antibody
摘要
Abstract
Connecting the hydroxyl of acid red 73 (AR73) molecule with a "spacer arm" containing a carboxyl as hapten, and the hapten was conjugated to bovine serum albumin (BSA) or ovalbumin (OA) by using N-hydroxysuccinimide active ester method as immunogen and coating antigen respectively, the polyclonal antibody titer was 2. 56×105 through immunization of New Zealand white rabbits, then an indirect ELISA procedure for the determination of acid red 73 was established. The half of inhibition concentration (IC50) was 181. 2 μg/L, the limit of detection for acid red 73 was 7. 9 μg/L, and the cross-reactivity study showed that the polyclonal antiserum was highly specific to AR73, and no cross-reactivity was detected between the obtained polyclonal antiserum and the other competitors except to Sudan red Ⅲ (1. 13%). The recoveries from the standards fortified blank samples were in the range of 63. 5% - 90. 7% with RSD lower than 6. 8%. The developed method can be used to determine the acid red 73 residue in shrimps, and can help to prepare the monoclonal antibody and develop the rapid test kits for acid red 73.关键词
酸性红73/酸性大红GR/多克隆抗体/活性酯法Key words
Acid Red 73/ Acid Red GR/ Polyclonal antibody/ Active ester method引用本文复制引用
常向彩,杨晓农,宋定州,刘倩,于学辉,程江,黄伟..酸性红73多克隆抗体的制备及其应用[J].分析化学,2012,40(10):1593-1597,5.基金项目
本文系西南民族大学研究生创新型科研重点项目(No.CX2011SZ16)和西南民族大学研究生学位点建设项目(No.2011XWD-S0906)资助 （No.CX2011SZ16）
