分析化学2012，Vol.40Issue(10)：1622-1626,5.DOI:10.3724/SP.J.1096.2012.20373
微流蒸发光散射检测器的研制及评价
Development and Evaluation of Micro Evaporative Light Scattering Detector
摘要
Abstract
A novel micro-flow evaporative light scattering detector (μELSD), suitable for capillary liquid chromatography (cLC) system, was constructed and eveluated. The main parameters of detector, such as the aperture of nebulizer, inner diameter of capillary, specification of the evaporative tube and scattering cell, were optimized. Under the optimal experimental conditions: nebulizer 410 μm I. D. , nebulized capillary 50 μm I. D. ×360 μm O. D. , evaporative tube 15 cm×4. 6 mm I. D. , scattering cell 60 mm×33 mm I. D. , the detection limitation of glucose (S/N>10) was 1 ng with a linear range of 0. 01 - 1 μg. Good reproducibility has been achieved in peak area and peak height with the RSD (w = 6) of 0. 4% and 0. 3% (n = 6) , respectively. The detector was successfully coupled to a cLC system and applied to the separation and detection of three kinds of sweeteners, in the condition of capillary column ( C18, 250 μm I. D. ) and 0. 1% (V/V) ammonium formate (pH = 4. 5): methanol (60:40, V/V) as mobile phase. The cLC-μELSD system has the advantages in universal applicability, high column efficiency, and less solvent and sample consumptions compare with a common HPLC-ELSD system, and is suitable for separation and analysis of real samples.关键词
蒸发光散射检测器/毛细管液相色谱/微型化/甜味剂Key words
Evaporative light scattering detector/ Capillary liquid chromatography/ Miniaturization/ Sweeteners引用本文复制引用
王玉红,刘芳,赵卉,王建民,王彦,阎超..微流蒸发光散射检测器的研制及评价[J].分析化学,2012,40(10):1622-1626,5.基金项目
本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072)、国家自然科学基金项目(No.21175092)和上海市引进技术的吸收与创新计划项目(No.JJ-YJCX-03-10-2586)资助 （No.2011YQ150072）
