分析化学2012，Vol.40Issue(11)：1661-1667,7.DOI:10.3724/SP.J.1096.2012.20176
高效液相色谱法同时测定软饮料中20种食品添加剂
Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector
摘要
Abstract
An efficient and accurate analytical method was developed for the simultaneous determination of 20 synthetic food additives, including acesulfame potassium, benzoic acid, sorbic acid, sodium saccharin, tartrazine, amaranthus red, ponceau 4R, caffeine, sunset yellow, aspartame, allure red, brilliant blue, erythrosine, methyl paraben, ethyl paraben, isopropyl paraben, n-propyl paraben, isobutyl paraben, n-butyl paraben and n-heptyl paraben, by high performance liquid chromatography (HPLC) coupled with photodiode array detector (PDA). A C18 stationary phase was used and the mobile phase contained an acetonitrile-methanol (10:90, V/V) mixture and 0. 1 mol/L ammonium acetate buffer solution at pH 7. 2. Successful separation was obtained for all the compounds within 50 min at 30 ℃by using gradient elution. The absorbance of 20 compounds behaved linearly with their concentrations ranged from 0. 10 mg/L to 300 mg/L with correlation coefficients more than 0. 998. The limits of detection of these compounds were from 0. 005 mg/L to 0. 110 mg/L. The recoveries were from 91. 4% to 100. 5% at spiked levels of 10-100 mg/L, The precision (n = 6) was range from 0. 6% to 4. 0%. The method has been used in the determination of 16 food additives in various drinks.关键词
食品添加剂/高效液相色谱/二极管阵列检测器/同时测定/软饮料Key words
Food additives/ Liquid chromatography/ Photodiode array detection/ Simultaneous determination/ Drinks引用本文复制引用
马康,蒋孝雄,赵敏,蔡冶强..高效液相色谱法同时测定软饮料中20种食品添加剂[J].分析化学,2012,40(11):1661-1667,7.基金项目
本文系国家质检总局食品安全专项(No.ASPAQ1010)和中国计量科学研究院基础科研费(No.AKY1216)资助 （No.ASPAQ1010）
