分析化学2012，Vol.40Issue(11)：1668-1673,6.DOI:10.3724/SP.J.1096.2012.20203
一种基于微流控芯片的生物样品循环给样富集方法
An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip
摘要
Abstract
A microfluidic chip based circular injection method for bio-samples was presented. The microfluidic chip with an annular peristaltic micropump and electromagnetic microvalves was fabricated using PDMS (polydimethylsiloxane) soft lithography process. The chip was then integrated with an immune probe chip immobilized with rabbit IgG, and realized the circular injection of Goat-anti-rabbit IgG (FITC labeled) solution and sample enrichment. Higher fluorescence intensity was obtained using circular injection than that using static adsorption, under different sample concentrations and 3 min binding time. By increasing the pumping speed from 130 μL/min to 300 u.L/min, the immune binding speed with 50 mg/L sample concentration was accelerated evidently, which meant the detection limit at a given time could be lower, but for 200 mg/L sample concentration, the effect was weaker. Compared to the continuous injection mode with about 1. 8 mL of sample consumption, only 7 μL sample was needed to realize the coincident results with the circular injection mode.关键词
微流体/微泵/循环/给样/富集Key words
Microfluidic/ Micropump/ Circulation/ Sample injection/ Enrichment引用本文复制引用
杜敏,叶雄英,冯金扬,马增帅,周兆英..一种基于微流控芯片的生物样品循环给样富集方法[J].分析化学,2012,40(11):1668-1673,6.基金项目
本文系国家重点基础研究发展计划(No.2007CB310504)和国家自然科学基金(No.50730009)资助项目 （No.2007CB310504）
