分析化学2013，Vol.41Issue(1)：10-14,5.DOI:10.3724/SP.J.1096.2013.20720
基于反相有机整体柱的新型蛋白质酶反应器
Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis
摘要
Abstract
X We developed a reversed phase monolithic column based enzyme reactor for protein analysis. Using the number of identified unique peptides, sequence coverage and the level of missed cleavage as the evaluation indicators, we systematically investigated the influence of buffer solution, porous structure of the column, digestion time and hydrophobicity of solid phase on the digestion performance of enzyme reactor. It had demonstrated that the reversed phase monolithic column based enzyme reactor had high digestion efficiency and might be further extended to the proteome analysis of complex samples.关键词
反相有机整体柱/蛋白质酶反应器/液相色谱-质谱联用Key words
Reversed phase polymer-based monolithic column/ Enzyme reactor/ Liquid chromatography coupled with mass spectrometry引用本文复制引用
刘静,王方军,张振宾,邹汉法..基于反相有机整体柱的新型蛋白质酶反应器[J].分析化学,2013,41(1):10-14,5.基金项目
本文系中国科学院科研装备研制项目(No.P020090520)和国家自然科学基金创新群体项目(No.21021004)资助 （No.P020090520）
