法医学杂志2013，Vol.29Issue(1)：17-20,4.DOI:10.3969/j.issn.1004-5619.2013.01.004
多重置换扩增在病理切片DNA分型中的应用
Applying Multiple Displacement Amplification to DNA Typing in the Pathological Section
摘要
Abstract
Objective To investigate the feasibility of applying multiple displacement amplification (MDA) to DNA typing in forensic pathological section. Methods Ninety-eight pieces of pathological sections were prepared in terms of 3 factors as the period of preservation,tissue types and death ages,and randomized into groups by Latin square by double 7-order design. Silicon bead method was used to extract the DNA template. Compared with the PCR amplification performed directly by AmpF€STR(R) IdentifierTM kit in the control group,MDA was performed before amplification in the experimental group. Based on the samples from fresh autopsies as the standard genotypes,the number of detection and the detection rate were analyzed and compared between the experimental group and the control group. Results Between the control group and the experimental group,there was significantly statistical difference regarding the rate of DNA typing in each period of the tissue sections preserved (P<0.01). The detection rate of the 16 loci in the experimental group was more than 95% when the period of the tissue sections were preserved within 360 d. There was significant difference in different tissue types (P<0.01). But there was no significant difference in different death ages (P>0.01). Conclusion MDA is efficacious in DNA typing of forensic pathological sections,for it can improve the DNA template quantification through abating the inhibiting factor's concentration of PCR and reducing the rate of allele drop out (ADO). However,the period of the sections preserved and tissue types would affect the results of genotyping by MDA.关键词
法医遗传学/冷冻切片/多重置换扩增Key words
forensic genetics/ frozen sections/ multiple displacement amplification分类
社会科学引用本文复制引用
张越,陈阳,杨元立,李继周,李朝品..多重置换扩增在病理切片DNA分型中的应用[J].法医学杂志,2013,29(1):17-20,4.基金项目
国家自然科学基金资助项目(C080804) （C080804）
安徽省自然科学基金资助项目(070413088) （070413088）
安徽省高校青年教师科研资助计划项目(2007jq1176) （2007jq1176）
