福州大学学报（自然科学版）2012，Vol.40Issue(6)：823-827,5.
八肋游仆虫中心蛋白的核磁共振研究
NMR studies on the Euplotes octocarinatus centrin
摘要
Abstract
The structural and Ca2+ binding properties of Euplotes octocarinatus Centrin (EoCen) and two fragments ( LC terminal domain ( EoCen - LC) and N terminal domain ( EoCen - N) ) were studied using solution NMR. The spectra of EoCen, EoCen - LC and EoCen - N domains were severely o-verlapped and highly disordered in the apo state, indicates a high conformational disorder, conforma-tional exchange or aggregation. Although the spectra of EoCen and LC terminal domain were improved in the presence of Ca2+ , the structures were not suitable for study using solution NMR. The N terminal domain (EoCen - N) become well structured in the presence of Ca2 + and amenable to structure determination by NMR.关键词
八肋游仆虫/中心蛋白/核磁共振/结构Key words
Euplotes octocarinatus/ centrin/ NMR/ structure分类
生物科学引用本文复制引用
洪晶,郭晨云,刘华,梁爱华,林东海,汪少芸..八肋游仆虫中心蛋白的核磁共振研究[J].福州大学学报（自然科学版）,2012,40(6):823-827,5.基金项目
福州大学科研启动基金资助项目(022304) （022304）
福州大学科技发展基金资助项目(2010-XQ-18) （2010-XQ-18）
福建省教育厅科研资助项目(JA11019) （JA11019）
