基于超高效液相色谱-四极杆飞行时间高分辨质谱的高通量血清代谢组学方法OA北大核心CSCDCSTPCD
Robust and High Throughput UPLC-QTOFMS Method for the Global Metabolic Profiling Study of Human Serum
采用超高效液相色谱-四极杆飞行时间高分辨质谱(UPLC-QTOFMS)联用技术,对血清前处理进行了考察和优化,并对液-质联用分析条件进行了优化,旨在建立用于血清中广谱小分子代谢物分析的高通量强耐用代谢组学方法,以期满足大批次生物样本数代谢组学研究的要求(样本数≥400个).通过考察不同有机溶剂沉淀蛋白对血清中蛋白的去除程度及38个代表性标准品化合物的提取效果,确定采用甲醇/乙腈(体积比1:9)沉淀蛋白法.血清和有机溶剂的体积比不小于1:4时,达到…查看全部>>
A robust and high throughput method was developed based on ultra performance liquid chromato-graphy and quadruple/time-of-flight mass spectrometry(UPLC-QTOFMS) for performing global metabolic profiling analysis on a large batch of human serum. Proteins in serum samples were precipitated by organic solvents. Using 38 reference standards, we compared serum preparation methods for protein precipitation by different organic solvents. Different conditions for glo…查看全部>>
成玉;刘玉敏;黄凤杰;陈天璐;郑晓皎;赵爱华;何品刚;贾伟
华东师范大学化学系,上海200062上海交通大学药学院,上海200240上海交通大学药学院,上海200240上海交通大学附属第六人民医院,上海200233上海交通大学药学院,上海200240上海交通大学药学院,上海200240华东师范大学化学系,上海200062Department of Nutrition,University of North Carolina at Greensboro,Kannapolis 28081,USA
化学化工
代谢组学血清前处理方法超高效液相色谱-四极杆飞行时间高分辨质谱(UPLC-QTOFMS)超声主成分分析
Global metabolic profiling Serum preparation method Ultra performance liquid chromatography and quadruple/time-of-flight mass spectrometry(UPLC-QTOFMS) Ultrasonic treatment Principal component analysis (PCA )
《高等学校化学学报》 2013 (1)
77-83,7
国家"九七三"计划项目(批准号:2007CB914700)资助.
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