广东医学2012，Vol.33Issue(23)：3519-3522,4.
2型糖尿病伴非酒精性脂肪性肝病大鼠肝脏UCP2的表达及意义
The expressions and significance of UCP2 in liver of rats with type 2 diabetes mellitus with nonalcohlic fatty liver disease
摘要
Abstract
Objective To investigate the expressions of UCP2 in liver of rats with long — term fat — enriched fed and lower dose of streptozocin — induced type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease ( NAFLD). Methods Thirty rats were randomly divided into normal groups, the NAFLD group, T2DM with NAFLD group. The subjects in normal control group were raised by standard animal feeds, while in NAFLD group by highly fat — enriched diet for 8 and 16 week, and in T2DM with NAFLD with plus lower dose of streptozocin ( 30 mg/kg). The livers were examined pathologically. The expressions of UCP2 in the liver were assessed by immunohistochemistry and semi — quantitative RT -PCR. The blood glucose, liver function, lipoprotein, and insulin were recorded at the 8th and 16th week. Results With the feeding times extended, extensive hydropic degeneration and varied steatosis were detected in the rat livers in NAFLD and T2DM with NAFLD groups, so were the significant elevation of blood glucose, insulin, liver enzymes, and lipids (P = 0. 002). There were also significant up - regulation of UCP2 protein and mRNA in both NAFLD and T2DM with NAFLD groups ( P 〈 0. 001) , while it was significantly more prominent in T2DM with NAFLD group ( P 〈 0. 001). Conclusion The expression of UCP2 in rats with T2DM and NAFLD, which present severe hepatic steatosis and inflamma-tion, is dramatically up — regulated, as compared with those with NAFLD alone.关键词
2型糖尿病/非酒精性脂肪性肝病/解偶联蛋白/胰岛素抵抗Key words
type 2 diabetes mellitus/ NAFLD rats/ uncoupling protein 2/ insulin resistance引用本文复制引用
武革,陈玉华,杨璐,胡桂芳,吴美芬..2型糖尿病伴非酒精性脂肪性肝病大鼠肝脏UCP2的表达及意义[J].广东医学,2012,33(23):3519-3522,4.基金项目
广东省医学科研基金资助项目(编号:A2004469) （编号:A2004469）
