广东医学2012，Vol.33Issue(23)：3523-3526,4.
骨髓间充质干细胞在体外肠上皮环境下免疫调节的作用机制
The immunoregulation mechanism of bone marrow mesenchymal stem cells on T lymphocyte in the existence of CaCo-2 in vitro
摘要
Abstract
Objective To investigate the immunoregulation mechanism of allogeneic bone marrow mesenchymal stem cells (BMMSCs) on T lymphocyte cell in the existence of CaCo -2. Methods BMMSCs and T lymphocyte cells of Wistar rats were prepared from bone marrow and spleen, respectively. Human colon carcinoma cell line ( CaCo -2) in good condition was selected as a model system for intestinal epithelium. Four groups were designed for experiment: experiment group (splenic lymphocytes/BMMSCs/CaCo -2 +ConA) , control group (splenic lymphocytes/CaCo -2 +ConA) , proliferation group (splenic lymphocytes + ConA) , and blank group (splenic lymphocytes). The vitality and the expression of TNF - α, IL - 10 and TGF -β were assessed at baseline, 24th and 48th hours. Results Compared with control group, the T lymphocyte cell proliferation was significantly reduced in experimental group, so was the TNF - α, while the IL - 10 and TGF -β were significantly elevated ( P 〈 0. 05 ). The regulatory T cells in experimental group were significantly increased than those in control group, though the activity of the regulatory T cell was significantly trimmed down with the extent of time ( P 〈 0. 05 ). Conclusion In the existence of CaCo - 2, T lymphocyte cell proliferation is reduced , so is the TNF -α.. But BMMSCs can secrete IL - 10 and TGF -β and boost the level of regulatory T cell to promote immune tolerance.关键词
骨髓间充质干细胞/T淋巴细胞/免疫调节/CaCo-2/共培养Key words
bone marrow mesenchymal stem cells/ T lymphocyte/ immunoregulation/ CaCo -2/ co -culture引用本文复制引用
杜杰静,宋红丽,沈中阳,郭瑞雪,吴本娟,付楠楠..骨髓间充质干细胞在体外肠上皮环境下免疫调节的作用机制[J].广东医学,2012,33(23):3523-3526,4.基金项目
天津市应用基础及前沿技术研究计划项目(编号:12JCZDJC25200,11JCZDJC27800),天津市卫生局科技基金面上项目(编号:2011KY11) （编号:12JCZDJC25200,11JCZDJC27800）
