广东医学2012，Vol.33Issue(24)：3691-3694,4.
七氟醚在亚麻醉浓度下对新生大鼠脑皮质凋亡和磷酸化JNK1/2、ATP合成酶β亚基表达的影响
The effects of Sevoflurane under sub-anesthetic concentration on the cortex cell apoptosis and expression of phosphorylated JNK1/2 and ATP synthase β subunit in new born rats
摘要
Abstract
Objective To investigate the effect of sevoflurane under 0. 5 MAC on cell apoptosis and expression of phosphorylated JNK1/2 and ATP synthase (3 subunit in new born rat cerebral cortex. Methods Sixty neonate rats (7 -day old) from 6 litters (10 pups from each litter) were randomly assigned into 4 groups. The rats were exposed Lo 1.8% sevoflurane or air as control for 4 h in sevoflurane groups and control groups, respectively. The blood sugar and blood gas assessments were carried out with cardiac puncture. After 2 - hour and 3- day recoveries, the brain tissues were obtained for assessment of phosphorylated JNK1/2, ATP synthase β subunit and Caspase - 3 using Western blot. Results Sevoflurane exposure did not affeet blood sugar and ABG result in neonatal rats, but Caused significantly temporary Caspase - 3 elevation by 419. 9% ( P 〈 0. 05) and reduction of ATP synthase β subunit by 39. 6% ( P 〈 0. 05) after 2 h recovery, which were all reversed after 3 d recovery. There was no significant change of phosphorylated JNK1/2. Conclusion sevoflurane exposure induces temporary apoptosis in neonatal rat cortex, probably via depression of ATP synthase β subunit.关键词
七氟醚/脑/细胞凋亡/ATP合成酶/c-Jun氨基末端激酶Key words
Sevoflurane/ brain/ apoptosis/ ATP synthase/ JNK引用本文复制引用
赵一凡,李玉娟,陈倩茹,曾敏婷..七氟醚在亚麻醉浓度下对新生大鼠脑皮质凋亡和磷酸化JNK1/2、ATP合成酶β亚基表达的影响[J].广东医学,2012,33(24):3691-3694,4.基金项目
国家自然科学基金资助项目(编号:30700787),广东省自然科学基金资助项目(编号:S2011010004558),广东省科技计划项目(编号:2010B060900039) （编号:30700787）
