广东医学2013，Vol.34Issue(1)：34-38,5.
表皮干细胞基质膜用于结膜重建的实验研究
A study of conjunctival reconstruction with epidermal progenitor cell-derived artificial conjunctiva sheet in rabbits
摘要
Abstract
Objective To determine the outcomes of conjunctival reconstruction by transplanting of epidermal progenitor cell - derived artificial conjunctiva in rabbits, further to evaluate the utility of artificial conjunctiva in ocular surface reconstruction. Methods A total of 20 New Zealand albino rabbits were divided into 4 groups in random. The bulbar conjunctiva in the right eye was removed and replaced with the artificial conjunctiva graft on the superior superficial sclera area. After 1, 2, 3 , and 4 weeks, the artificial compound membranes were collected. Immunohistochemistry with human K13, MUC5A/C antibody corresponding to the specific surface marker to human conjunctival epithelium, and PAS stain were carried out. Results After 2 weeks of the transplantation, positive human K13 antibody and human MUC5A/C antibody were observed on compound membranes. Furthermore, positive PAS was observed on goblet cell on the compound membranes. Conclusion The epidermal progenitor cell - derived artificial conjunctiva can be used as a new substitute for clinical conjunctival reconstruction.关键词
表皮干细胞/结膜重建/复合基质膜Key words
dpidermal progenitor cell/ conjunctiva reconstruction/ artificial conjunctiva引用本文复制引用
孟繁剑,陈家祺..表皮干细胞基质膜用于结膜重建的实验研究[J].广东医学,2013,34(1):34-38,5.基金项目
国家自然科学基金资助项目(编号:30271388),"十五"国家科技攻关计划项目(编号:国科发计字[2004]437号) （编号:30271388）
