广东医学2013，Vol.34Issue(4)：505-508,4.
5-氮杂-2'-脱氧胞苷对HepG2肝癌细胞生物学行为的影响
The effects of 5-Aza-2′-deoxyetidine on the biological behaviors of human hepatocarcinoma cell line HepG2.
摘要
Abstract
Objective To investigate the effects and mechanism of 5 - Aza - 2' - deoxyetidine ( 5 - Aza - CdR ) on the biological behaviors of human hepatocarcinoma cell line HepG2. Methods The HepG2 cells were treated with 50 μmoL/ 5 - Aza - CdR for 72h. The cell cycle and apoptosis were assessed by flow cytometry ( FCM ) with annexin -FITC/PI staining, while the invasive ability was examined by using transwell invasion assay. The mRNA expression of tumor suppressor gene SIAH1 was detected by using reverse transcription polymerase chain reaction ( RT - PCR ) before and after 5 - Aza - CdR treatment. Results After 5 - Aza - CdR treatment, significant increase of S phase cell count was observed with FCM analysis ( P < 0. 05 ), indicating cell cycle blockage in HepG2 cells; meanwhile, significant elevation of apoptosis rate, reduction in invasive activity and up - regulation of SIAH1 were also revealed ( P <0. 05 ). Conclusion The 5 - Aza - CdR regulates cell cycle and induces apoptosis, as well as inhibits the cell invasion, through up - regulation of the expression of SIAH1.关键词
5-氮杂-2'-脱氧胞苷/肝癌/细胞周期/凋亡/SIAH1Key words
5 - Aza - 2' - deoxyetidine/ hepatocellular carcinoma/ cell cycle/ apoptosis/ SIAH1引用本文复制引用
刘瑶,罗耀玲,黄文峰,王小农,黄才斌..5-氮杂-2'-脱氧胞苷对HepG2肝癌细胞生物学行为的影响[J].广东医学,2013,34(4):505-508,4.基金项目
国家自然科学基金资助项目(编号:81000908),江西省自然科学基金资助项目(编号:2010GQY0036) （编号:81000908）
