广东药学院学报2012，Vol.28Issue(6)：588-592,5.DOI:10.3969/j.issn.1006-8783.2012.06.002
提高牛血清白蛋白聚乳酸聚乙醇酸微球包封率的处方筛选
Preparation optimization about bovine serum albumin entrapment efficiency of poly lactic acid glycolic acid microspheres
摘要
Abstract
Objective To study the mechanism of different influence factors on the entrapment efficiency of poly lactic acid glycolic acid (PLGA) microspheres, and optimize the preparation of microspheres with high entrapment efficiency. Methods A modified double-emulsion method was chosen,which was based on the principal that sodium alginate combined with calciumion produces ionic interaction to form a kind of sustained-release gel. Bovine serum albumin ( BSA ) was used as model drug, while PLGA as carrier material. PLGA concentration in organic phase, different rotational speed and time during the emulsifying process,different solidifying time,volume and calcium chlorine concentration in external water phase 2 and sodium alginate concentration in internal water phase had been studied to value the influence on the entrapment efficiency. Results It has been proved that PLGA concentration in organic phase, different rotational speed and time during the emulsifying process, volume and calcium chlorine concentration in external water phase 2 could affect the entrapment efficiency apparently. Different solidifying time and sodium alginate concentration in the internal water phase showed little difference. Entrapment efficiency of microspheres produced from modified prescribing technology was (90. 78 ±0. 06)%. Conclusion This paper optimized preparation conditions of BSA PLGA microspheres and improved the encapsulation efficiency of microspheres effectively.关键词
蛋白微球/聚乳酸聚乙醇酸/海藻酸钠/包封率Key words
protein microsphere/poly lactic acid glycolic acid/sodium alginate/entrapment efficiency分类
医药卫生引用本文复制引用
李姝瑾,罗宇燕,黎呐,杜鹏阳,张永明..提高牛血清白蛋白聚乳酸聚乙醇酸微球包封率的处方筛选[J].广东药学院学报,2012,28(6):588-592,5.基金项目
广东省重大专项科技计划项目(2011A080504003) （2011A080504003）
