广东药学院学报2012，Vol.28Issue(6)：603-607,5.DOI:10.3969/j.issn.1006-8783.2012.06.005
酚红法和改良重量法分别研究葛根素的大鼠在体肠吸收机制
Transport of puerarin in rat intestine in situ by modified gravimetry and phenol red assay
摘要
Abstract
Objective To study the rat intestine absorption mechanism of puerarin by in situ single pass perfusion, and compare the difference of gravimetry and phenol red assay. Methods The rat single pass perfusion technique was applied. Gravimetry and phenol red assay were used to correct the perfusion volume. The concentration of puerarin was determined by HPLC. A series of studies were carried out including the absorption at different concentrations(100,200 and 400 (μg o mL-1 ) and at different intestinal regions. Results Phenol red assay's parameters were almost all of negative values. These results went against common sense. Gravimetry's results showed that puerarin could be absorbed in all rat intestines without special absorption sites and the differences were negligible. There was no highest saturated concentration raised with the increasing concentration of puerarin. Conclusion Gravimetry was more suitable than phenol red assay for studying the absorption mechanism of puerarin.关键词
葛根素/在体单向肠灌流法/吸收机制/重量法/酚红法Key words
puerarin /in situ single pass perfusion/absorption mechanism/gravimetry/phenol red assay分类
医药卫生引用本文复制引用
黄嗣航,龙晓英,袁飞,陈莉,蔡宝玲,邱慧琳..酚红法和改良重量法分别研究葛根素的大鼠在体肠吸收机制[J].广东药学院学报,2012,28(6):603-607,5.基金项目
国家自然科学基金面上项目(30973953C1909) （30973953C1909）
