中国妇幼健康研究2013，Vol.24Issue(1)：105-108,4.DOI:10.3969/j.issn.1673-5293.2013.01.039
检测羊水中神经源性细胞辅助诊断NTD的研究
The study on detection of neurogenic cells in amniotic fluid in aided diagnosis of NTD
摘要
Abstract
Objective To explore the relationship between rapidly adhering cell ( RAC ) and neural tuber defect ( NTD ) by studying the differences in morphology and adhesion rate of live cells, and to explore the correlation between positive expression rate of neurogenic cells in amniotic fluid and NTD. Methods The amniotic fluid from pregnant women who did prenatal diagnosis ( confirmed with normal fetus by FISH ) in outpatient of Gansu Provincial Maternity and Child-care Hospital and that from women confirmed with NTD were cultured. The differences in morphology and adhesion rate between normal amniotic fluid group ( control group ) and NTD group ( experimental group ) were observed. Then the neuron-specific marker of anti-MAP2 and anti-NeuN were selected to identify the positive expression rates of neuron-specific markers in control group ( n = 15 ) and experimental group ( n = 11 ) through immunofluorescence. Results Adhesion rate of live cells of the experimental group was significantly higher than that of control group and the difference was significant t = 8. 909, P = 0.000 ). Immunofluorescence results revealed negative expression of neurogenic cells in the control group and blank group. The expression of anti-NeuN was positive and expression of anti-MAP2 was negative in NTD group. The differences were statistically significant between control group and experimental group(χ2 =7. 717, P = 0. 021 ). Conclusion Detection of the neurogenic cells in amniotic fluid can assist the diagnosis of NTD.关键词
神经管畸形/羊水培养/免疫荧光/神经细胞Key words
neural tube defect ( NTD )/ amniotic fluid culture/ immunofluorescence/ nerve cells分类
医药卫生引用本文复制引用
孙庆梅,林晓娟,刘婷婷,姜岩,赵媛,吴菊,宋筱玉,代维斯..检测羊水中神经源性细胞辅助诊断NTD的研究[J].中国妇幼健康研究,2013,24(1):105-108,4.基金项目
甘肃省科技厅科技支撑资助项目(0708NKCA104) （0708NKCA104）
