国际病理科学与临床杂志2012，Vol.32Issue(6)：479-487,9.DOI:10.3969/j.issn.1672-7347.2012.06.004
不同转移潜能鼻咽癌细胞株的差异膜蛋白质组研究
Differential membrane proteomic study of nasopharyngeal carcinoma cell lines with different metastatic potentials
陈瑜 1李娇阳 1李茂玉 1肖志强1
作者信息
- 1. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙410008
- 折叠
摘要
Abstract
Objective: To screen metastasis-related membrane proteins of nasopharyngeal carcinoma (NPC). Methods: After enriching membrane proteins from high metastatic NPC cell line 5-8F and non-metastatic NPC cell line 6-10B, the post-digestion isotope 18O labeling method, followed by Q-TOF-MS/MS, was conducted to identify the differentially expressed membrane proteins in the 2 cell lines. The expression of differential membrane protein EphA2 was measured by Western blot. Bioinformatics analysis was used to reveal the biological functions and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: A total of 31 differential membrane proteins were identified, of which 25 proteins were up-regulated in 5-8 F cells and 6 proteins were down-regulated. One of the differential proteins, EphA2, was further confirmed by Western blot. Bioinformatics analysis showed that the functions of the differential membrane proteins were mainly involved in cell adhesion, receptor recycling, and cell junction. The differential membrane proteins were also involved in 14 KEGG pathways including cell adhesion, cell junction, and cell migration pathways. Conclusion: Thirty-one differentially expressed membrane proteins may play an important role in NPC invasion and metastasis, which will provide a novel clue for further investigating the molecular mechanisms of NPC metastasis.关键词
鼻咽癌/转移/膜蛋白质组/定量蛋白质组学/18O标记Key words
nasopharyngeal carcinoma/ metastasis/ membrane proteome/ quantitative proteomics/ 18 O labeling引用本文复制引用
陈瑜,李娇阳,李茂玉,肖志强..不同转移潜能鼻咽癌细胞株的差异膜蛋白质组研究[J].国际病理科学与临床杂志,2012,32(6):479-487,9.
