神经损伤与功能重建2012，Vol.7Issue(6)：395-399,5.DOI:10.3870/sjsscj.2012.06.002
安脑丸对急性脑出血大鼠OX42、脑源性神经营养因子及突触素表达的影响
Effect of An Nao Wan on the Expression of OX42,BDNF and SYN in Rats after Intracerebral Hemorrhage
梁慧 1梅元武1
作者信息
- 1. 华中科技大学同济医学院附属协和医院神经内科,武汉430022
- 折叠
摘要
Abstract
Objective: To study the effects of An Nao Wan (ANW) on the expression of OX42,BDNF and SYN in rats after intracerebral hemorrhage. Methods: One hundred and ninety SD rats were randomly divided into normal control group (n=10), sham operated group, model group and ANW treatment group (n = 60 respectively). Experimental intracerebral haematoma was made by injecting Ⅶ type collagenase into the right globus pallidus of rats in the model group and the ANW treatment group. The rats in the ANW treatment group were treated by ANW. Immunohistochemical staining and western blot analysis were employed to detect the expression of OX-42, BDNF and SYN at l2 h,ld,2d,4d,7d or 10 d after the operation. Results: The numbers of OX42 and BDNF positive cells and the expression of BDNF and SYN protein in the model group and the ANW treatment group were increased significantly when compared with those in the normal control group and the sham operated group (P<0. 01). Compared with those in the model group, the number of OX42 positive cells was decreased significantly while the number of BDNF positive cells and the expression of BDNF and SYN protein were increased significantly in the ANW treatment group (P<0. 01). Conclusion: The activation of microglia and the expression of BDNF and SYN were involved in the pathological process of intracerebral hemorrhage. ANW treatment could inhibit the activation of microglia and increase the expression of BDNF and SYN protein after intracerebral hemorrhage.关键词
安脑丸/脑出血/小胶质细胞/脑源性神经营养因子/突触素Key words
An Nao Wan/ intracerebral hemorrhage/ microglia/ BDNF/ SYN分类
医药卫生引用本文复制引用
梁慧,梅元武..安脑丸对急性脑出血大鼠OX42、脑源性神经营养因子及突触素表达的影响[J].神经损伤与功能重建,2012,7(6):395-399,5.
