南方农业学报2012，Vol.43Issue(12)：1916-1921,6.DOI:10.3969/j:issn.2095-1191.2012.12.1916
华南杂交稻亲本米质性状配合力的SSR标记鉴定
Identification of SSR markers linked with quality grain traits and combining ability of elite hybrid rice parents in Southern China
摘要
Abstract
[Objective]The present experiment was conducted to screen for SSR markers linked with quality grain traits and combining ability of elite hybrid rice parental lines in Southern China area in order to provide basis for the selection and improvement of parental hybrid rice quality. [Method]Utilizing the SSR marker technology combined with the incomplete diallel crosses method, the molecular markers of quality grain traits correlation and combining ability in 61 F, traits of 18 hybrid rice parents in Southern China area were identified. [Result]From analyzing the 3 traits of brown rice rate, rice grain length, and grain length-to-width ratio, the correlation coefficients between the F, and male sterile lines and restorer lines were similar. The correlation coefficient between F, and restoring lines was significantly greater than that of F, and male sterile lines when the relevant chalky white grain rate, chalkiness degrees, alkali spreading values, and amylose contents were compared; at the same time, the correlation coefficient between the parents' genetic distance and F, was relatively high. The 17 parent SSR molecular markers obtained were significantly related to these traits. Amongst the parent SSR molecular markers, there were 6 containing amylose contents, which were RM5, RM250, RM274, RM549, RM336, and RM427. Also from the parent SSR molecular markers, there were 5 containing alkali digestion value, which were RM236, RM18, RM274, RM336, and RM427. Two other parent SSR molecular markers contained chalky grain rate, which were RM5 and RM276. The remaining parent SSR markers each contain one of the following: brown rice rate, head rice rate, chalkiness degree, and gel consistency, which was respectively RM274, RM246, RM276, and RM549. [Conclusion]SSR marker screening could be used directly for forecasting the hybrid rice grain quality in Southern China and for improving the combination ability of molecular assisted breeding.关键词
杂交稻/米质/配合力/SSR标记/相关分析/华南地区Key words
hybrid rice/ rice quality/ combining ability/ SSR marker/ correlation analysis/ Southern China分类
农业科技引用本文复制引用
黄永相,郭建夫,王慧,陈志强..华南杂交稻亲本米质性状配合力的SSR标记鉴定[J].南方农业学报,2012,43(12):1916-1921,6.基金项目
广东省重大科技专项项目(2007A020400003) （2007A020400003）
广东省现代农业产业技术体系建设专项资金项目(财教389号) （财教389号）
