南方农业学报2012，Vol.43Issue(12)：1927-1933,7.DOI:10.3969/j:issn.2095-1191.2012.12.1927
白玉蔗组织培养研究
Tissue culture of Baiyu chewing cane
摘要
Abstract
[Objective]Using the Baiyu chewing cane collected from Guigang City, Guangxi as testing material, the main factors impacting Baiyu chewing cane in vitro culture was explored in order to establish the rapid regeneration propagation system. [Method]After using the tissues from Baiyu chewing cane's slightly-tailing leaves as explant callus for bud differentiation culture of callus, adventitious bud proliferation, and plantlet browning prevention and rooting induction were conducted using different culture medium and hormone combinations. [ Result ]Baiyu chewing cane leaf slicing for callus induction was a convenient and effective method. After the whorl sections were connected with the culture medium (MS+2,4-D 3 mg/L, agar 5 g/L, sugar 30 g/L) for 30 d, explants rapidly expanded, the edges became granular, light yellow or yellow, and the embryogenic callus or paste callus displayed uniformed quality. The optimum culture medium for buds differentiation induction in Baiyu chewing cane was MS+6-BA 1.0 mg/L+ NAA0.2 mg/L. For the proliferation of adventitious buds, the optimum effect resulted from the MS+6-BA 3.0 mg/L+KT 0.5 mg/L+NAA 0.6 mg/L+ sugar 30.0 g/L treatment. To prevent browning effects, the PVP and activated carbon tissue culture seedlings had different effects; the 1% activated carbon could effectively inhibit the proliferation of adventitious shoots browning in Baiyu chewing cane culture medium. In the rooting culturing process, the optimal culturing medium was MS+ NAA 0.6 mg/L+ sugar 50.0 g/L + activated carbon 0.1%. [Conclusion]By the orthogonal design test, the regeneration system of Baiyu chewing cane breeding was established in order to speed up the breeding rate of Baiyu chewing cane, improve propagation coefficient, and provide an effective way for capital-saving.关键词
白玉蔗/组织培养/愈伤组织/芽分化/增殖Key words
Baiyu chewing cane/ tissue culture/ callus/ bud initiation/ proliferation分类
农业科技引用本文复制引用
余坤兴,杨柳,刘俊仙,李松,方锋学,杨丽涛,李杨瑞..白玉蔗组织培养研究[J].南方农业学报,2012,43(12):1927-1933,7.基金项目
广西自然科学基金项目(2012jjBA30008) （2012jjBA30008）
广西农业科学院基本业务专项项目(桂农科2012YZ14) （桂农科2012YZ14）
广西甘蔗研究所基本业务专项项目(G2010016) （G2010016）
广西农业科学院博士启动基金项目 （）
广西农业科学院科技发展基金项目[200805(自)] （自）
广西甘蔗研究所基本科研业务专项项目(G2009015) （G2009015）
