南方农业学报2012，Vol.43Issue(12)：1958-1962,5.DOI:10.3969/j:issn.2095-1191.2012.12.1958
植物染色体标本的制备和染色体核型分析研究进展
Research progress in plant chromosome samples preparation and karyotype analysis
摘要
Abstract
Chromosome is the genetic material carrier. Plant chromosome and karyotype analysis technology is the most common and useful method in cytogenetics research, and is widely used in species relationship identification, chromosome variation, and hybrid analysis. The improved plant chromosome preparation method in recent years were reviewed from included sample selection, material pretreatment, material regulation, material dissociation, chromosome preparation, staining and observation, etc. The karyotype analysis technology was introduced by traditional manual analysis, pho-toshop software analysis, automatic karyotype analysis, flow cytometry, etc. The developing future directions for plant karyotype analysis are as follows: improving the production technology to obtain clearer metaphase chromosome images, standardizing the unified chromosome standards equipment and establishing a unified standard to facilitate results comparison for benefiting plant taxonomy and genetic research. Also, the data statistics and analysis methods were improved in order to reduce the experimental cost.关键词
植物染色体/标本/核型分析/研究进展Key words
plant chromosome/ sample/ karyotype analysis/ research progress分类
农业科技引用本文复制引用
丁鸿,邱东萍,陈少雄..植物染色体标本的制备和染色体核型分析研究进展[J].南方农业学报,2012,43(12):1958-1962,5.基金项目
揭阳职业技术学院重点科研项目(JYCKZ0503) （JYCKZ0503）
