南方农业学报2012，Vol.43Issue(12)：1968-1972,5.DOI:10.3969/j:issn.2095-1191.2012.12.1968
板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用
Inhibition effect of Castanea mollissima Blume bract extract on intestinal bacteria in Dendrolimu kikuchii Matsumura
摘要
Abstract
[Objective]This research studied the inhibition effect of Castanea mollissima Blume bract extract on intestinal bacteria in Dendrolimu kikuchii Matsumura in order to create new biological pesticides developed from plant sources and to provide theoretical basis for pesticide development. [Method]Using 10 strains of intestinal bacteria extracted from age 3 and age 5 Dendrolimu kikuchii Matsumura instar larvae as test strains, the inhibition effect of different organic Castanea mollissima Blume bract extract solvents was determined using the Oxford cup method. [Result]Different organic solvents of Castanea mollissima Blume bract extract had some inhibition effects on intestinal bacteria in Dendrolimu kikuchii Matsumura. As the quality concentration increased, the inhibition effect became more noticeable; different extractions were arranged in the following rank from the strongest inhibition rate to the weakest inhibition rate: acetic ether> ethanol>chloroform>butyl alcohoblight petroleum. Ethyl acetate extract exhibited strong inhibition activity on the intestinal bacteria of Dendrolimu kikuchii Matsumura. The minimum inhibitory concentration (MIC) was 6.250 mg/mL, and the inhibition effect of petroleum ether extract was not obvious or non-existent. [ Conclusion ] Castanea mollissima Blume bract extract could be developed as the new plant biological source pesticide to control Dendrolimu kikuchii Matsumura.关键词
板栗苞壳/提取物/思茅松毛虫/肠道细菌/抑菌作用Key words
Castanea mollissima Blume bract/ extract/ Dendrolimus kikuchii Matsumura/ intestinal bacteria/ inhibition effect分类
农业科技引用本文复制引用
刘绍雄,王明月,王金华,张敬宜,思斯,熊智..板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用[J].南方农业学报,2012,43(12):1968-1972,5.基金项目
云南省自然科学基金面上项目(2009CD073) （2009CD073）
云南省中青年学术技术带头人后备人才培养项目(2010CI016) （2010CI016）
云南省森林灾害预警与控制重点实验室开放基金项目(ZK09A102) （ZK09A102）
西南林业大学大学生创新基金项目(1138,1146) （1138,1146）
西南林业大学生物技术特色专业建设项目(50116001) （50116001）
