南方农业学报2012，Vol.43Issue(12)：1976-1980,5.DOI:10.3969/j:issn.2095-1191.2012.12.1976
采用CFDA荧光染色技术监测木薯渣堆肥中的细菌动态
Bacteria detection in cassava residue compost by CFDA fluorescent staining technique
摘要
Abstract
[Objective]The present study aimed to determine the efficiency of CFDA fluorescent staining technique on detecting thermophilic bacteria in order to clarify the bacteria quantity changes during cassava residue compost and to provide a basis for regulating and evaluating the composing process. [ Method ]Both thermophilic and mesophilic bacteria were detected by CFDA fluorescent staining under 30℃ and 60℃ , and their staining effects were observed. Both the CFDA technique and plate count method were adopted simultaneously to monitor the dynamic changes in the quantity and vertical distribution of bacteria in cassava residue compost. [ Result ]The CFDA technique could identify thermophilic bacteria effectively under 60℃. Data from CFDA technique showed that, during the cassava residue compost detection, mesophilic bacteria were quantitatively predominant at early stage, but decreased as the time of detection increased; on the other hand, the amount of thermophilic bacteria increased rapidly as time increased and became the predominant bacteria variety at the late fermentation stage. The highest temperature was detected when the compost pile measured at the depth of 10-40 cm, in which the thermophilic bacteria were predominant; in the bottom half of the compost pile, the temperature decreased, pH level turned acidic, and mesophilic bacteria became the predominant bacteria variety. [ Conclusion ]Ther-mophilic bacteria were the predominant microbial group in the high temperature compost of cassava residue, and the CFDA technique could be used for the accurate evaluation of the bacteria group.关键词
木薯渣/堆肥/CFDA技术/细菌Key words
cassava residue/ compost/ CFDA technique/ bacteria分类
农业科技引用本文复制引用
吴圣进,韦仕岩,王灿琴,汪茜,覃晓娟,陈廷速..采用CFDA荧光染色技术监测木薯渣堆肥中的细菌动态[J].南方农业学报,2012,43(12):1976-1980,5.基金项目
广西自然科学基金项目(2010GXNSFD003) （2010GXNSFD003）
广西农业科学院科技发展基金项目(2010Z003) （2010Z003）
