南方农业学报2012，Vol.43Issue(12)：2079-2082,4.DOI:10.3969/j:issn.2095-1191.2012.12.2079
鸡传染性法氏囊病病毒抗血清的研制
Antiserum development for chicken infectious bursal disease virus
摘要
Abstract
[Objective]This research developed high performing and safe chicken infectious bursal disease (IBD) prevention and treatment products to ensure the high efficiency of chicken infectious bursal disease virus (IBDV) antiserum and to solve the safety and immunological stress problems at the same time. [ Method ] Using SPF chicken as the immune animal, the corresponding IBDV antiserum was prepared according to the requirements from《Veterinary Biological Products Technical Guidance and Principle Experimental Studies 》 and 《Veterinary Pharmacopoeia of P.R. China》(2010 Ed). After detoxification treatment, the semi-finished and finished antiserums underwent production inspections. [Result] For the antiserums obtained from IBDV NJ immunized SPF chickens at 4-weeks old, the serum titer before detoxification treatment was 5≥1:64, while the serum titer after detoxification treatment was 5≥1:32. From the test results of semi-finished and finished serums, the antiserum products after detoxification treatment were orange-hued, sterile, without mycoplas-ma, and without exogenous virus; the formaldehyde and thimerosal residues also did not exceed the prescribed standards, which were safe to use in mice, guinea pigs, and SPF chickens. [Conclusion]The IBDV antiserum products, developed according to the standards of (Veterinary Pharmacopoeia of P. R. China), could be used for emergency IBD prevention and early IBD treatment. The antiserum could also be listed as a formal sales product in the future to meet market demands.关键词
鸡传染性法氏囊病/抗血清/治疗/SPF鸡/脱毒处理Key words
chicken infectious bursal disease/ antiserum/ treatment/ SPF chickens/ virus-free treatment分类
农业科技引用本文复制引用
王占伟,邵国青,刘茂军,冯志新,王丽,高云飞,周开华,尹秀凤..鸡传染性法氏囊病病毒抗血清的研制[J].南方农业学报,2012,43(12):2079-2082,4.基金项目
江苏省农业科技自主创新资金项目[CX(11)2037] （11）
