南方农业学报2013，Vol.44Issue(1)：6-11,6.DOI:10.3969/j:issn.2095-1191.2013.1.6
利用SSR分子标记研究苦瓜资源的遗传多样性
Genetic diversity analysis in bitter gourd germplasm resources based on SSR molecular markers
摘要
Abstract
[Objective]The present experiment was conducted to study the genetic diversity and relationship amongst different bitter gourds (Monodrama charantia L.) varieties in order to provide reference for the innovation and cultivation of new bitter gourd varieties. [Method]Using 16 pairs of SSR primers and the SSR molecular markers technology, the genetic diversity of 50 bitter gourd samples was examined and the cluster analysis was performed. [Result]A total of 90 al-leles were amplified with 16 SSR primers, and the average values of polymorphism loci percentage (P) , genetic heterozygosity (He), effective number of allcles (Ne), Shannon-Wiener index (H'), polymorphism information content (PIC), and genetic similarity coefficient were 97.78%, 0.853, 7.728, 2.087, 0.834, and 0.706, respectively. The UPG-MA cluster analysis results showed that the 50 bitter guard samples could be clustered into 6 groups. The second group included 18 samples and 2 subclasses, which was the most genetically abundant and diverse group with the largest number of subjects. The second group also contained 3 types of balsam pear. [ Conclusion ] Since the genome was complex and the genetic information was ample amongst the 50 tested bitter gourd materials, directional continuity breeding played important roles in the accumulation of bitter gourd traits.关键词
苦瓜/SSR分子标记/遗传多样性/亲缘关系Key words
bitter gourd (Monodrama charantia L.)/ SSR molecular markers/ genetic diversity/ genetic relationship分类
农业科技引用本文复制引用
李光光,郑岩松,李向阳,张华,郭培国,黄红弟..利用SSR分子标记研究苦瓜资源的遗传多样性[J].南方农业学报,2013,44(1):6-11,6.基金项目
广州市农业局招标项目(GZCQC1102FG04007) （GZCQC1102FG04007）
广州市科技支撑项目(2009Z1-E801) （2009Z1-E801）
广州市科技攻关项目(2011Y2-00015) （2011Y2-00015）
