南方农业学报2013，Vol.44Issue(1)：23-27,5.DOI:10.3969/j:issn.2095-1191.2013.1.23
马铃薯优良品种大西洋、云薯201茎尖诱导技术
Stem apex induction technology for excellent potato varieties Daxiyang and Yunshu 201
摘要
Abstract
[Objective]This research aimed to master the stem apex induction technology for excellent potato varieties Daxiyang and Yunshu 201 in order to provide technical reference for the virus-free and rapid propagation of factory potatoes. [Method]The stem apex of excellent potato varieties Daxiyang and Yunshu 201 were used as test subjects, then the influential effects of the sterilization, different basic culture media, plant hormone type, and concentration ratio on the stem apex induction of potato were explored. [Result]Among different sterilant combinations of 2 factors and 3 factors, the optimum combination of buds sterilization was using 75% alcohol 30 s+10% NaClO 5 min+0.1% HgCl2 for 5 min. The basic MS culture medium was better than other basic culture media for bud induction. In the culture medium induced bud, adding 0.02 mg/L GA3 could improve the bud induction rate, and the optimum hormone treatment combination was 6-BA 0.5 mg/L + NAA 0.01 mg/L + GA3 0.02 mg/L. [ Conclusion ] Suitable stem apex induction system for potato varieties Daxiyang and Yunshu 201 was constructed to set the foundation for industrialized potato seedlings.关键词
马铃薯/茎尖诱导/激素组合/灭菌剂Key words
potato/ stem apex induction/ hormone combination/ sterilant分类
农业科技引用本文复制引用
唐燕梅,陆飞,韦鹏宵,岑秀芬,李全,陆春霞,梁贵秋..马铃薯优良品种大西洋、云薯201茎尖诱导技术[J].南方农业学报,2013,44(1):23-27,5.基金项目
广西农业重点科技计划项目(NK200822) （NK200822）
