南方农业学报2013，Vol.44Issue(1)：145-149,5.DOI:10.3969/j:issn.2095-1191.2013.1.145
鸡白痢沙门氏菌冻干菌种保存试验
Preservation experiment on frozen-dried Salmonella Pullorum strains
摘要
Abstract
[Objective]The survival rate and biological characteristics of different aged frozen-tried Salmonella pullorum strains were determined in order to provide basic references for the conservation and utilization of Salmonella pullorum strain resources. [ Method ]For Salmonella pullorum frozen-dried bacteria strains preserved under -20℃ for 15-34 years, the survival rate was determined, the morphology and biochemical characteristics were identified, and the serotype, virulence, and antigenicity of the colony type were detected, respectively. [Result]The results showed that froze-dried strains stored at -20℃ for 15-34 years still have a relatively high survival rates, and the culture characteristics, morphology and biochemical characteristics, and antigenicity between the initial strains and froze-dried strains were not significant. The mutational rate of colony types and serotypes was 8.3%-14.9% and 4.2%-8.7%, respectively. The froze-dried strains could recover its original virulence by rejuvenation through susceptible animals.[Conclusion]Using the freeze-drying method for Salmonella pullorum strains preservation produced high survival rate, and there were no significant biological activity differences between the initial strains and the frozen-dried strains. These findings showed that freeze-drying storage method was suitable for long-term preservation of Salmonella Pullorum.关键词
鸡白痢沙门氏菌/冻干菌种/存活率/变异率/抗原性/毒力Key words
Salmonella Pullorum/ freeze-drying strains/ survival rate/ mutational rate/ antigenicity/ virulence分类
农业科技引用本文复制引用
龚建森,刘学贤,许明,窦新红,刘加圣,徐步,王鑫,俞燕,戴有理..鸡白痢沙门氏菌冻干菌种保存试验[J].南方农业学报,2013,44(1):145-149,5.基金项目
江苏省农业自主创新项目[CX(11)3023] （11）
江苏省农业三新工程项目(SXGC[2012]383) （SXGC[2012]383）
江苏省农业科技支撑项目(BE2010449) （BE2010449）
