南方农业学报2013，Vol.44Issue(1)：155-160,6.DOI:10.3969/j:issn.2095-1191.2013.1.155
广西仔猪腹泻病毒病原流行病学调查
Investigation on epidemiology of piglets diarrhea virus disease in Guangxi
摘要
Abstract
[Objective]This research aimed to understand the main pathogens and molecular epidemiological characteristics of piglets diarrhea virus found in Guangxi in order to provide theoretical basis for piglet diarrhea prevention and control. [Method]Through the RT-PCR method, the porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) , and porcine rotavirus (PoRV) from 232 tissue samples collected from the pig farms in 11 cities of Guangxi from 2011 to July 2012 were investigated. Parts of the PEDV positive M gene materials were cloned and analyzed along with the alignments and phylogenetic trees of some M gene sequences from other domestic and international provinces and cities. [Result]From the 232 tissue samples collected from the 11 cities of Guangxi, the positive rates of PEDV and PoRV were respectively 37.50% and 0.03%, yet the TGEV tested negative. For part of the PEDV positive samples, 14 M genes were obtained after all the M genes were amplified, and the nucleotide homologies of the deduced amino acid sequences were respectively 97.4%-100.0% and 96.0%-100.0%. Comparing and analyzing the 14 PEDV amplified M genes with the 40 PEDV M genes domestically and internationally reported in GenBank, 13 M genes were discovered to be located on a large branch (G1 ), out of which 9 were located on the same branch G1 -1 as the 5 reference strains isolated and obtained from Guangdong, Sichuan, and Jiangsu in 2011 and Thailand in 2008, and 2 reference strains isolated and obtained from South Korea in 2007. Other 4 M genes were located on the same branch G1-2 as the reference strain JS-2004-2 isolated and obtained from Jiangxi, Jiangsu, Guangdong, and Fujian in 2011land Jiangsu in 2004. The last PEDV M gene (NNA -11), unlike the others, was located on G2, which was relatively far away from thr other M genes. [Conclusion] From 2011 until now, the main causes for piglet diarrhea in Guangxi was PEDV, in which the infection degree was different in varying cities, and presence of the infection was year-round. The most prevalent strains in Guangxi exhibited higher affinity with the four Chinese provinces of Guangdong, Fujian, Jiangsu, and Jiangxi since 2011 , and the strains were also very closely related to strains found in Thailand and South Korea since 2007, but the stains were far apart from Northern strains, classic vaccinated strains, and Korean vaccinated strains found and isolated since 2006.关键词
仔猪腹泻病/猪流行性腹泻病毒/猪传染性胃肠炎病毒/猪轮状病毒/流行病学/M基因/广西Key words
piglet diarrhea/ porcine epidemic diarrhea virus (PEDV)/ transmissible gastroenteritis virus (TGEV)/ porcine rotavirus (PoRV)/ molecular epidemiology/ M gene/ Guangxi分类
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郭旋,黄伟坚,陈静,刘欢,侯韶毅,龙凤,李莹莹,曾娟,兰家暖,刘芳..广西仔猪腹泻病毒病原流行病学调查[J].南方农业学报,2013,44(1):155-160,6.基金项目
广西科学研究与技术开发计划项目(桂科攻1123007-3) （桂科攻1123007-3）
